Double Stained Fish

Stickleback and pipefish that have been double stained with Alcian Blue (blue - staining cartilage) and Alizarin Red (red - staining bone). Staining technique can be found here: (Walker and Kimmel, 2007).

Figure 1 Developing dorsal fin and "plates" of a young pipefish

Figure 2 Developing facial bones and cartilages in stickleback.

Figure 3 Developing facial bones and cartilages in pipefish.